RESUMEN
Dengue virus serotype 2 (DENV-2) is responsible for dengue epidemics on a global scale and is associated with severe cases of the disease. This study conducted a phylogenetic investigation of DENV-2 isolates from 2017 to 2021 originating from the northern states of Brazil. A total of 32 samples from DENV-2 isolates were analyzed, including 12 from Acre, 19 from Roraima, and one from Tocantins. Only one lineage of the Asian-American genotype and one lineage of the cosmopolitan genotype were observed: Lineage 1, Asian-American genotype (connection to Puerto Rico); Lineage 5, cosmopolitan genotype (connection to Peru). Our results provide important data regarding the study of DENV genotypes and lineage distribution and open up possibilities for probable introduction and dissemination routes.
RESUMEN
Dengue virus 2 (DENV-2) seriously contributes to dengue-related mortality. It includes five nonsylvatic genotypes, with cosmopolitan being the most widespread with a significant contribution to the total number of DENV-2 cases globally. In South America, the cosmopolitan genotype was first recorded in 2019 in Madre de Dios, Peru, and then in Goiás (Midwest Brazil) in November 2021. In this study, we tested 163 human serum samples from Acre (Northern Brazil) collected during a DENV outbreak between 2020 and 2021 for all DENV genotypes by RT-qPCR. Of the 163 samples, 139 were positive for DENV-2, and 5 were positive for DENV-1. Five DENV-2-positive samples from early 2021 were sequenced, and the sequences clustered with the three other DENV-2 cosmopolitan genotype sequences already recorded on the continent. These results create a geographical link, suggesting the possible route of introduction of the DENV-2 cosmopolitan genotype into Brazil through the border with Peru, from which it may have dispersed to Midwest Brazil.
RESUMEN
Objetivo: Descrever o processo de desenvolvimento de uma webtecnologia educacional, do tipo blog, sobre citologia clínica, para estudantes e profissionais da área da saúde. Métodos: Pesquisa metodológica para construção de tecnologia educacional em saúde, desenvolvida em três etapas: Revisão da literatura (2019), a partir da qual se deu o delineamento teórico; Fotodocumentação (2020), que foi a fase responsável pela obtenção do conteúdo imagético; Construção (2020-2021), a partir da qual se deu a criação do blog. Resultados: Na primeira fase, obteve-se a fundamentação teórica. Na segunda fase, realizou-se a obtenção das imagens e na terceira fase, o desenvolvimento do produto técnico-tecnológico. Conclusão: O blog, emerge como modelo de suporte para proporcionar um caráter de ubiquidade à informação e como consequência disso, ampliar o potencial gerador de conhecimento sobre citologia clínica entre estudantes e profissionais de diversos campos da área da saúde, com enfoque à Biomedicina e Enfermagem(AU)
Objective: To describe the development process of an educational webtechnology, like a blog, about clinical cytology, for students and health professionals. Methods: Methodological research for the construction of educational technology in health, developed in three stages: Literature review (2019), from which the theoretical outline was given; Photodocumentation (2020), which was the phase responsible for obtaining the image content; Construction (2020-2021), from which the creation of the blog took place. Results: In the first phase, the theoretical foundation was obtained. In the second phase, the images were obtained and in the third phase, the development of the technical-technological product. Conclusion: The blog emerges as a support model to provide a ubiquitous character to information and, as a consequence, expand the potential generator of knowledge about clinical cytology among students and professionals from different fields of health, with a focus on Biomedicine and Nursing(AU)
Objetivo: Describir el proceso de desarrollo de una tecnología web educativa, como un blog, sobre citología clínica, para estudiantes y profesionales de la salud. Métodos: Investigación metodológica para la construcción de tecnología educativa en salud, desarrollada en tres etapas: Revisión de la literatura (2019), a partir de la cual se dio el esquema teórico; Fotodocumentación (2020), que fue la fase encargada de obtener el contenido de la imagen; Construcción (2020-2021), a partir de la cual tuvo lugar la creación del blog. Resultados: En la primera fase se obtuvo el fundamento teórico. En la segunda fase se obtuvieron las imágenes y en la tercera fase, el desarrollo del producto técnico-tecnológico. Conclusión: El blog surge como un modelo de apoyo para dotar de un carácter ubicuo a la información y, como consecuencia, ampliar el potencial generador de conocimiento sobre citología clínica entre estudiantes y profesionales de diferentes campos de la salud, con enfoque en Biomedicina y Enfermería(AU)
